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US 2024/0301472 A1Published application
Nucleic acid detection method, compound and fluorescent probe
AssigneeWakayama University
InventorTakashi SAKAMOTO
Life of this patent
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Abstract
A compound suitable for nucleic acid detection or viscosity measurement is provided. A compound of the disclosure includes a donor and three acceptors bound to the donor so as to perform rotary motion with respect to the donor. A fluorescence property of the compound changes in accordance with change in the rotary motion of each acceptor.
Claims15
A method comprising:
- using a compound having a structure represented by general formula (1A) or general formula (1B), for nucleic acid detection,
- where
- RA, RB, and RC each independently have a structure selected from the group consisting of formula (1C), formula (1D), formula (1E), and formula (1F) below (* representing a binding site),
- X is selected from the group consisting of O, S, and Se,
- R1, R3, R5, and R7 are each independently selected from the group consisting of H, —(CH2)n-, and —(CH2CH2O)n-, n being a natural number of 1 to 100, and
- R2, R4, R6, and R5 are each independently selected from the group consisting of H, —OH, methyl, an amine, a terminal alkyne, an alkene, an alkyl acid, an amine acid, sulfonate (SO3−), an aryl group, and a heterocyclic compound.
3 more elements
A compound having a structure represented by general formula (1A) or general formula (1B),
- where
- RA, RB, and RC each independently have a structure selected from the group consisting of formula (1C), formula (1D), formula (1E), and formula (1F) below (* representing a binding site),
- X is selected from the group consisting of O, S, and Se,
- R1, R3, R5, and R7 are each independently selected from the group consisting of —(CH2)n-, and —(CH2CH2O)n-, n being a natural number of 1 to 100, and
- R2, R4, R6, and R5 are each independently selected from the group consisting of H, —OH, methyl, an amine, a terminal alkyne, an alkene, an alkyl acid, an amine acid, sulfonate (SO3−), an aryl group, and a heterocyclic compound.
2 more elements
A compound comprising:
- a donor;
- and
- three acceptors bound to the donor so as to perform rotary motion with respect to the donor, wherein
- a fluorescence property of the compound changes in accordance with change in the rotary motion of each acceptor.
1 more element
Dependencies are inferred from the claim text.