Recombinant zymomonas mobilis for producing ethylene glycol, method and uses thereof
AssigneeHubei University
InventorsShihui YANG, Xiongying YAN, Mian LI, Xia WANG, Qiaoning HE
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Abstract
Recombinant Zymomonas mobilis for producing ethylene glycol, method and uses thereof are provided. The recombinant Zymomonas mobilis carries and expresses genes related to a synthesis pathway of xylonic acid and genes related to a synthesis pathway of ethylene glycol.
Claims3
A recombinant strain from Zymomonas mobilis for producing ethylene glycol, wherein the recombinant strain is transformed with a recombinant plasmid, the recombinant plasmid carries a connected sequence of a gene xdh, a gene yagF, a gene yagE;
- and a gene ZMO1771 wherein a nucleotide sequence of the gene xdh is the nucleotide sequence set forth in SEQ ID NO. 1, a nucleotide sequence of the gene yagF is the nucleotide sequence set forth in SEQ ID NO. 9, a nucleotide sequence of the gene yagE is the nucleotide sequence set forth in SEQ ID NO. 10, and a nucleotide sequence of the gene ZMO1771 is the nucleotide sequence set forth in SEQ ID NO. 15.
A method of preparing a recombinant strain from Zymomonas mobilis for producing ethylene glycol comprising:
- preparing a recombinant plasmid for producing ethylene glycol, the recombinant plasmid carries a connected sequence of a gene xdh, a gene yagF, a gene yagE and a gene ZMO1771 wherein a nucleotide sequence of the gene xdh is the nucleotide sequence set forth in SEQ ID NO. 1, a nucleotide sequence of the gene yagF is the nucleotide sequence set forth in SEQ ID NO. 9, a nucleotide sequence of the gene yagE is the nucleotide sequence set forth in SEQ ID NO. 10, and a nucleotide sequence of the gene ZMO1771 is the nucleotide sequence set forth in SEQ ID NO. 15, wherein the gene xdh is expressed under a control of a promoter Ppde, wherein the gene yagF, the gene yagF, and the gene ZMO1771 are linked by a sequence of RBS and expressed under the control of a promoter Peno;
- and
- transforming Zymomonas mobilis with the recombinant plasmid.
Dependencies are inferred from the claim text.