Methods and yeast cells for production of desaturated compounds
AssigneeFMC Agricultural Solutions A/S
InventorsCarina Holkenbrink, Irina Borodina, Baojian Ding, Christer Löfstedt
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Abstract
The present invention relates to yeast cells capable of producing Δ13 desaturated fatty acyl-CoAs and optionally desaturated fatty alcohols, said yeast cells expressing heterologous Δ13 desaturases. Said compounds are precursors of sex pheromone components of several insects, such as the navel orangeworm Amyelois transitella, the grape leaffolder Desmia funeralis, Cedar processionary moth Thaumetopoea bonjeani, the iron prominent Notodonta dromedarius, and others.
Claims22
A pheromone composition comprising a Z13-fatty alcohol, (Z11, Z13)-hexadecadien-1-ol, a Z13-fatty alcohol acetate, (Z11, Z13)-hexadecadien-1-ol acetate, a Z13-fatty aldehyde and/or (Z11, Z13)-hexadecadienal, wherein said pheromone composition comprises at least 70% biobased carbon, such as at least 75% biobased carbon, such as at least 80% biobased carbon, such as at least 85% biobased carbon, such as at least 90% biobased carbon, such as at least 95% biobased carbon, such as 100% biobased carbon.
A pheromone compound selected from the group consisting of a Z13-fatty alcohol, (Z11, Z13)-hexadecadien-1-ol, a Z13-fatty alcohol acetate, (Z11, Z13)-hexadecadien-1-ol acetate, a Z13-fatty aldehyde and/or (Z11, Z13)-hexadecadienal, wherein said pheromone compound comprises at least 70% biobased carbon, such as at least 75% biobased carbon, such as at least 80% biobased carbon, such as at least 85% biobased carbon, such as at least 90% biobased carbon, such as at least 95% biobased carbon, such as 100% biobased carbon.
A yeast cell capable of producing a desaturated fatty acyl-coenzyme A (fatty acyl-CoA) having n′ double bond(s), wherein at least one of said double bond(s) is at position 13,
- wherein said yeast cell expresses a heterologous Δ13 fatty acyl-CoA desaturase, said desaturase being capable of introducing a double bond at position 13 in a saturated fatty acyl-CoA or a desaturated fatty acyl-CoA having a carbon chain consisting solely of single and double bonds, preferably a desaturated fatty acyl-CoA, wherein said fatty acyl-CoA has a carbon chain length of at least 14 and has n double bond(s),
- wherein n and n′ are integers,
- wherein 0≤n≤3 and wherein 1≤n′≤4.
The yeast cell according to any one of the preceding claims, wherein n′=2.
Use of an Amyelois transitella desaturase in a method for introducing a double bond at position 13 in a saturated or desaturated fatty acyl-CoA, optionally wherein the Amyelois transitella desaturase is AtrAATQ (SEQ ID NO:
- 1) or functional variants thereof having at least 70% similarity or identity thereto, such as at least 80%, such as at least 90%, such as at least 91%, such as at least 92%, such as at least 93%, such as at least 94%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% similarity or identity thereto.
Use of an Notodonta dromedarius desaturase in a method for introducing a double bond at position 13 in a saturated or desaturated fatty acyl-CoA, optionally wherein the Notodonta dromedarius desaturase is NdDes1 (SEQ ID NO:
- 56) or functional variants thereof having at least 70% similarity or identity thereto, such as at least 80%, such as at least 90%, such as at least 91%, such as at least 92%, such as at least 93%, such as at least 94%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% similarity or identity thereto.
Use of an Amyelois transitella desaturase in a method for introducing a double bond at position 13 in a saturated fatty acyl-CoA or a desaturated fatty acyl-CoA having a carbon chain consisting solely of single and double bonds, optionally wherein the Amyelois transitella desaturase is AtrAATQ (SEQ ID NO:
- 1) or functional variants thereof having at least 70% similarity or identity thereto, such as at least 80%, such as at least 90%, such as at least 91%, such as at least 92%, such as at least 93%, such as at least 94%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% similarity or identity thereto.
Use of an Notodonta dromedarius desaturase in a method for introducing a double bond at position 13 in a saturated fatty acyl-CoA or a desaturated fatty acyl-CoA having a carbon chain consisting solely of single and double bonds, optionally wherein the Notodonta dromedarius desaturase is NdDes1 (SEQ ID NO:
- 56) or functional variants thereof having at least 70% similarity or identity thereto, such as at least 80%, such as at least 90%, such as at least 91%, such as at least 92%, such as at least 93%, such as at least 94%, such as at least 95%, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99%, such as 100% similarity or identity thereto.
Dependencies are inferred from the claim text.