Engineered microorganism for improved ethanol fermentation

2:

• The recombinant host cell of claim 1, wherein the cell is capable of reduced glycerol production compared to the same cell without the heterologous polynucleotide encoding the glycerol transporter when fermented under the same conditions.

3:

• The recombinant host cell of claim 1, wherein the heterologous polynucleotide encoding the non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) is operably linked to a promoter that is foreign to the polynucleotide.

4:

• The recombinant host cell of claim 1, wherein the non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) has a mature polypeptide sequence with at least 80% sequence identity to any one of SEQ ID NOs:
• 262-280 and 365-391.

(canceled)

6:

• The recombinant host cell of claim 1, wherein the non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) has a mature polypeptide sequence comprising or consisting of the amino acid sequence of any one SEQ ID NOs:
• 262-280 and 365-391.

8:

• The recombinant host cell of claim 1, wherein the glycerol transporter has a mature polypeptide sequence with at least 80% sequence identity to any one of SEQ ID NOs:
• 312-323.

(canceled)

10:

• The recombinant host cell of claim 1, wherein the glycerol transporter has a mature polypeptide sequence comprising or consisting of the amino acid sequence of any one of SEQ ID NOs:
• 312-323.

11:

• The recombinant host cell of claim 1, wherein the cell comprises an active pentose fermentation pathway.

12:

• The recombinant host cell of claim 1, wherein the cell further comprises a heterologous polynucleotide encoding a glucoamylase.

13:

• The recombinant host cell of claim 1, wherein the cell further comprises a heterologous polynucleotide encoding an alpha-amylase.

14:

• The recombinant host cell of claim 1, wherein the cell further comprises a disruption to an endogenous gene encoding a glycerol 3-phosphate dehydrogenase (GPD) and/or a disruption to an endogenous gene encoding a glycerol 3-phosphatase (GPP).

15:

• The recombinant host cell of claim 1, wherein the cell is capable of higher ethanol production compared to the same cell without the heterologous polynucleotide encoding the glycerol transporter under the same conditions.

16:

• The recombinant host cell of claim 1, wherein the cell is capable of higher ethanol production compared to the same cell without the heterologous polynucleotide encoding the non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) when fermented under the same conditions.

(canceled)

18:

• The recombinant host cell of claim 1, wherein the cell is a Saccharomyces cerevisiae cell.

19:

• A composition comprising the recombinant host cell of claim 1 and one or more naturally occurring and/or non-naturally occurring components, such as components are selected from the group consisting of:
• surfactants, emulsifiers, gums, swelling agents, and antioxidants.

20-21. (canceled)

23:

• The method of claim 22, wherein saccharification of step (a) occurs on a starch-containing material, and wherein the method comprises liquefying the starch-containing material by contacting the material with an alpha-amylase prior to saccharification.

(canceled)

25:

• The method of claim 22, wherein fermentation and saccharification are performed simultaneously in a simultaneous saccharification and fermentation (SSF).

26:

• The method of claim 22, wherein the fermentation product is ethanol.

27-29. (canceled)