Plant Vectors, Compositions and Uses Relating Thereto
AssigneesUniversity of Maryland, College Park, The Regents of the University of California
InventorsAnne Elizabeth Simon, Jingyuan Liu, Georgios Vidalakis, Sohrab Bodaghi
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Abstract
The present disclosure relates to a single stranded RNA vector suitable for introducing a therapeutic agent, such as a peptide, a protein or a small RNA, into a host plant. The vector does not encode for any movement protein or coat protein, but is capable of systemic and phloem-limited movement and replication within the host plant.
Claims23
A ribonucleic acid (RNA) vector comprising a heterologous segment(s), wherein said heterologous segment(s) is attached to the RNA vector through a scaffold structure.
2-13. (canceled)
An RNA vector comprising a truncated hairpin relative to a corresponding wild-type RNA molecule, and a heterologous segment(s) coupled to said truncated hairpin.
An RNA vector derived from citrus yellow vein associated virus (CYVaV) having the nucleic acid sequence of SEQ ID NO:1 and comprising a heterologous segment(s) at position 2250, 2301, 2319, 2330, 2331, 2336, 2375 or 2083 thereof.
An RNA vector comprising a heterologous segment(s) comprising a hairpin having a sequence on one side complementary to a sequence within Citrus tristeza virus (CTV).
A plant comprising a sour orange rootstock and an iRNA-based vector comprising a heterologous segment(s) that targets a nucleic acid of Citrus tristeza virus (CTV).
A method for introducing a heterologous segment(s) into a host plant comprising introducing into said host plant an RNA-based vector a) after encapsidating the RNA vector in a capsid protein other than the capsid protein of CVEV, or b) by agroinfiltration after inoculating an agroinfiltration site with Xanthomonas citri subsp. citri (Xcc).
30-32. (canceled)
An RNA vector comprising an siRNA or other non-coding RNA effective against a plant pathogenic bacteria.
36-38. (canceled)
Dependencies are inferred from the claim text.