Display substrate and method for manufacturing same, and display device

A manufacturing method of a display substrate, comprising:

• forming a plurality of sub-pixels on a main surface of a base substrate, wherein the sub-pixel comprises a light-emitting element, the light-emitting element has a light-emitting region, the light-emitting element comprses a first electrode, a light-emitting functional layer, and a second electrode, the second electrode is located on a side of the light-emitting functional layer facing away from the base substrate, the first electrode is located on a side of the light-emitting functional layer close to the base substrate, and the light-emitting functional layer comprses a plurality of sub-functional layers;
• and
• forming a separation structure between light-emitting regions of adjacent sub-pixels, wherein forming the separation structure comprises forming a first separation part and a second separation part which are stacked, and the first separation part is located on a side of the second separation part close to the base substrate,
• wherein the second separation part has a protruding part, the protruding part protrudes relative to a side of the first separation part close to the second separation part, at least one sub-functional layer of the light-emitting functional layer is disconnected at the protruding part, a material of the first separation part comprises an organic material, a material of the second separation part comprises an organic material, and along a direction from the first electrode to the second electrode, an orthographic projection of the separation structure on the base substrate gradually decreases and then gradually increases.