US 2024/0266003 A1 · DETERMINING AND REMOVING INTER-CLUSTER LIGHT INTERFERENCE

Abstract

This disclosure describes embodiments of methods, systems, and non-transitory computer readable media that accurately estimates the crosstalk from an adjacent cluster of oligonucleotides onto a target cluster of oligonucleotides and removes or reduces the crosstalk emitted by the adjacent cluster of oligonucleotides from the target cluster of oligonucleotides. For instance, the disclosed systems can detect the intensity values for a target cluster and the adjacent cluster. Based on the intensity values of the adjacent cluster, the disclosed systems can determine an inter-cluster-interference metric that estimates the crosstalk emitted from the adjacent cluster. The disclosed systems can remove the inter-cluster-interference metric from the intensity value of the target cluster and generate modified intensity values for the target cluster.

Claims20

Dependent claims

A system comprising:

at least one processor;
and
a non-transitory computer readable medium comprising instructions that, when executed by the at least one processor, cause the system to:
detect, for a sequencing cycle, a first set of intensity values for a first signal from a first cluster of oligonucleotides and a second set of intensity values for a second signal from a second cluster of oligonucleotides;
determine a set of illumination indicators representing whether the first cluster of oligonucleotides is illuminated during the sequencing cycle based on the first set of intensity values;
determine an inter-cluster-interference metric estimating light interference from the first cluster of oligonucleotides on the second cluster of oligonucleotides based on the set of illumination indicators;
and
generate, for the sequencing cycle, a modified second set of intensity values for the second signal from the second cluster of oligonucleotides by removing the inter-cluster-interference metric from the second set of intensity values.

5 more elements

A non-transitory computer-readable medium storing instructions thereon that, when executed by at least one processor, cause a computing device to:

detect, for a sequencing cycle, a first set of intensity values for a first signal from a first cluster of oligonucleotides and a second set of intensity values for a second signal from a second cluster of oligonucleotides;
determine a set of illumination indicators representing whether the first cluster of oligonucleotides is illuminated during the sequencing cycle based on the first set of intensity values;
determine an inter-cluster-interference metric estimating light interference from the first cluster of oligonucleotides on the second cluster of oligonucleotides based on the set of illumination indicators;
and
generate, for the sequencing cycle, a modified second set of intensity values for the second signal from the second cluster of oligonucleotides by removing the inter-cluster-interference metric from the second set of intensity values.

2 more elements

A method comprising:

detecting, for a sequencing cycle, a first set of intensity values for a first signal from a first cluster of oligonucleotides and a second set of intensity values for a second signal from a second cluster of oligonucleotides;
determining a set of illumination indicators representing whether the first cluster of oligonucleotides is illuminated during the sequencing cycle based on the first set of intensity values;
determining an inter-cluster-interference metric estimating light interference from the first cluster of oligonucleotides on the second cluster of oligonucleotides based on the set of illumination indicators;
and
generating, for the sequencing cycle, a modified second set of intensity values for the second signal from the second cluster of oligonucleotides by removing the inter-cluster-interference metric from the second set of intensity values.

2 more elements

Dependencies are inferred from the claim text.

Determining and removing inter-cluster light interference

Life of this patent

Determining and removing inter-cluster light interference

Life of this patent

Abstract

Claims20