Compositions and methods for enhanced protein production in bacillus cells
AssigneeDanisco US Inc.
InventorsRyan L. Frisch, Zhen Ma, Hongxian He, Brian Paul
Life of this patent
Estimating the term…
Abstract
Certain embodiments of the disclosure are related to recombinant Bacillus strains comprising enhanced protein productivity phenotypes, compositions and methods for constructing such recombinant Bacillus cells, and the like. More particularly, the recombinant Bacillus strains described herein are particularly useful for the enhanced production of proteins of interest when grown/cultivated/fermented under suitable conditions.
Claims21
A recombinant Bacillus sp, cell comprising an introduced polynucleotide comprising at least 85% sequence identity to the nucleic acid sequence of SEQ ID NO:
- 16.
A recombinant Bacillus sp, cell derived from a parental Bacillus sp, cell producing a protein of interest (POI), wherein the recombinant cell comprises an introduced polynucleotide encoding a phosphatidylserine synthase (PssA) protein comprising at least 85% sequence identity to SEQ ID NO:
- 17 and produces an increased amount of the POI relative to the parental cell when cultivated under the same conditions.
A recombinant Bacillus sp, cell derived from a parental Bacillus sp, cell comprising a wild-type pssA gene encoding a native phosphatidylserine synthase (PssA) protein, wherein the recombinant cell comprises an introduced heterologous promoter sequence which replaces the wild-type pssA gene promoter sequence of the parental cell.
(canceled)
An expression cassette comprising an upstream (5′) promoter sequence operably linked to a downstream (3′) open reading frame (ORF) sequence encoding a PssA protein comprising at least 85% sequence identity to SEQ ID NO:
- 17, and optionally comprising a downstream (3′) terminator sequence operably linked to the upstream (5′) ORF.
(canceled)
A method for producing an increased amount of a protein of interest (POI) comprising:
- (a) obtaining or constructing a parental Bacillus cell producing a POI and modifying the cell by introducing therein a polynucleotide encoding a phosphatidylserine synthase (PssA) protein comprising at least 85% sequence identity to SEQ ID NO:
- 17, and
- (b) cultivating the modified cell under suitable conditions for the production of the POI,
- wherein the modified cell produces an increased amount of the POI relative to the parental cell when cultivated under the same conditions.
Dependencies are inferred from the claim text.