Compositions, kits, and methods for detecting sti pathogen sequences

A composition for amplifying target sequences from sexually transmitted infection (STI) pathogen genomes at about 99% strain coverage for each pathogen, the composition comprising:

• (a) a first set of forward primers and reverse primers for amplifying one or more target sequences present in target regions of the Chlamydia trachomatis (CT) genome;
• (b) a second set of forward primers and reverse primers for amplifying one or more target sequences present in target regions of the Mycoplasma genitalium (MG) genome;
• (c) a third set of forward primers and reverse primers for amplifying one or more target sequences present in target regions of the Neisseria gonorrhoeae (NG) genome;
• and
• (d) a fourth set of forward primers and reverse primers for amplifying one or more target sequences present in target regions of the Trichomonas vaginalis (TV) genome,
• wherein the composition provides about 99% strain coverage for each pathogen.