Partitioning cells for high throughput single-cell sequencing
AssigneesChan Zuckerberg Biohub, Inc., The Regents of the University of California
InventorsAdam Abate, Xiangpeng Li, Benjamin Demaree
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Abstract
The present disclosure provides materials and methods for partitioning cells and high throughput, single-cell multi-omic sequencing. Methods for pathogen detection and identification, microbiome analysis, personalized medicine, environmental analysis where single-cell information is critical are each provided herein.
Claims45
A method of sequencing one or more nucleic acids from a single cell comprising the steps of:
- (a) preparing single cells for coding comprising compartmentalizing a population of cells in permeable compartments comprising single cells;
- (b) barcoding nucleic acid molecules associated with the single cells, wherein a unique barcode is used for each single cell;
- and
- (c) sequencing one or more nucleic acids from a single cell.
The method of any of the preceding claims, wherein cells are selected from mammalian cells, bacterial cells, fungal cells, yeast cells, and plant cells.
The method of any of the preceding claims, wherein the one or more nucleic acids are selected from the group consisting of DNA, genomic DNA, RNA and mRNA.
Dependencies are inferred from the claim text.