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US 2024/0254538 A1Published application
Particles associated with oligonucleotides
AssigneeBecton, Dickinson and Company
InventorsGretchen Yinbon Lam, Eleen Shum
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods, compositions, and kits for barcoding nucleic acids. For example, particles for barcoding are provided, where the particle comprises: a first plurality of oligonucleotide barcodes each comprising a target-binding region, and a second plurality of oligonucleotides each comprising a target-specific target-binding region.
Claims33
-78. (canceled)
A method for determining the numbers of targets in a sample, comprising:
- barcoding copies of a first plurality of targets using a first plurality of oligonucleotide barcodes associated with a synthetic particle to generate copies of a first plurality of barcoded targets;
- barcoding copies of a second plurality of targets in a sample using a second plurality of oligonucleotide barcodes associated with the synthetic particle to generate copies of a second plurality of barcoded targets,
- wherein each of the first plurality of oligonucleotide barcodes and the second plurality of oligonucleotide barcodes comprises an identical cellular label sequence and a molecular label sequence,
- wherein at least 10 of the first plurality of oligonucleotide barcodes and the second plurality of oligonucleotide barcodes comprise different molecular label sequences,
- wherein each of the first plurality of oligonucleotide barcodes comprises a target-binding region, and
- wherein each of the second plurality of oligonucleotide barcodes comprises a target-specific target-binding region;
- and
- estimating the number of each of the first plurality of targets and the second plurality of targets using the molecular label sequences of the first plurality of oligonucleotide barcodes and the second plurality of oligonucleotide barcodes.
5 more elements
-88. (canceled)
A method for capturing targets in a sample, comprising:
- hybridizing copies of a first plurality of targets using a first plurality of oligonucleotide barcodes associated with a synthetic particle to generate copies of a first plurality of barcoded targets;
- hybridizing copies of a second plurality of targets using a second plurality of oligonucleotide barcodes associated with the synthetic particle to generate copies of a second plurality of barcoded targets,
- wherein each of the first plurality of oligonucleotide barcodes and the second plurality of oligonucleotide barcodes comprises an identical cellular label sequence and a molecular label sequence,
- wherein at least 10 of the first plurality of oligonucleotide barcodes and the second plurality of oligonucleotide barcodes comprise different molecular label sequences,
- wherein each of the first plurality of oligonucleotide barcodes comprises a target-binding region, and
- wherein each of the second plurality of oligonucleotide barcodes comprises a target-specific target-binding region.
3 more elements
-95. (canceled)
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-105. (canceled)
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-120. (canceled)
-208. (canceled)
A method for determining the numbers of targets in a sample, comprising:
- barcoding copies of a plurality of targets in a sample using a plurality of oligonucleotide barcodes associated with a synthetic particle to generate copies of a plurality of barcoded targets,
- wherein each of the plurality of oligonucleotide barcodes comprises an identical cellular label sequence, a molecular label sequence, and a target-binding region, and wherein at least 10 of the plurality of oligonucleotide barcodes comprise different molecular label sequences,
- wherein the synthetic particle comprises a first plurality of spacer oligonucleotides, and wherein each of the first plurality of spacer oligonucleotides does not comprise the target-binding region;
- and
- estimating the number of each of the plurality of targets in the sample using the molecular label sequences of the plurality of oligonucleotide barcodes.
2 more elements
-213. (canceled)
A method for capturing targets in a sample, comprising:
- hybridizing copies of a plurality of targets in a sample using a plurality of oligonucleotide barcodes associated with a synthetic particle to generate copies of a plurality of barcoded targets,
- wherein each of the plurality of oligonucleotide barcodes comprises an identical cellular label sequence, a molecular label sequence, and a target-binding region, and wherein at least 10 of the plurality of oligonucleotide barcodes comprise different molecular label sequences,
- wherein the synthetic particle comprises a first plurality of spacer oligonucleotides, and wherein each of the first plurality of spacer oligonucleotides does not comprise the target-binding region.
-265. (canceled)
Dependencies are inferred from the claim text.