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AssigneesCathay Biotech Inc., Cibt America Inc.
InventorsWenbo LIU, Min XU, Chen YANG, Xiucai LIU
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The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.
A mutated promoter or a variant thereof, wherein the mutated promoter or a variant thereof comprises a nucleic acid sequence having at least 80% sequence identity to SEQ ID NO:
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