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US 2024/0254474 A1Published application
Methods, systems, and kits for dual indexing nucleic acids
Assignee10x Genomics, Inc.
InventorCorey M. NEMEC
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Abstract
While robust multiplexed high-throughput systems allow for widespread use of genome-wide chromatin accessibility (e.g., using ATAC-seq) and other next-generation sequencing studies at single cell resolution, misassignment of index molecules between samples hampers the accuracy of the sequencing result. The present disclosure provides kits, methods, and compositions for dual-indexing to allow for accurate assignment of sequencing reads to their original source samples.
Claims20
A method for detecting nucleic acid index hopping, comprising:
- (a) appending index sequences to a plurality of nucleic acid molecules, wherein:
- (i) a first nucleic acid molecule of the plurality of nucleic acid molecules comprises a first index sequence and a second index sequence;
- and (ii) the first index sequence and the second index sequence are different index sequences;
- (b) obtaining sequence reads from the first nucleic acid molecule, wherein sequencing reads obtained from the first nucleic acid molecule comprise an index sequence different from the first index sequence and the second index sequence;
- and
- (c) determining that the first nucleic acid molecule, prior to or during (b), was subject to index hopping from the sequencing reads obtained from the first nucleic acid molecule.
3 more elements
Dependencies are inferred from the claim text.