Variants of terminal deoxynucleotidyl transferase and uses thereof
AssigneesDNA Script, Institut Pasteur, Centre National De La Recherche Scientifique
InventorsElise CHAMPION, Mikhael SOSKINE, Thomas YBERT, Marc DELARUE
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Abstract
The present invention relates to variants of Terminal deoxynucleotidyl Transferase (TdT), each of which (i) has an amino acid sequence similarity to SEQ ID NO: 2, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 with corresponding amino acid substitutions, (ii) is capable of synthesizing a nucleic acid fragment without a template and (iii) is capable of incorporating a modified nucleotide into the nucleic acid fragment.
Claims14
A variant of Terminal deoxynucleotidyl Transferase (TdT) which (i) comprises an amino acid substitution selected from C173G/R/P/A/V/S/N/Q/D wherein the position is numbered by reference to the amino acid sequence set forth in SEQ ID NO:2, (ii) is capable of synthesizing a nucleic acid fragment without template, and (iii) is capable of incorporating a modified nucleotide into the nucleic acid fragment.
Dependencies are inferred from the claim text.