Cell culture medium and supplements for corneal and skin cell culture
OwnerChe John CONNON, Ricardo GOUVEIAinventors
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Abstract
The present invention provides novel cell culture media and supplements for use in serum-free or reduced-serum cell culture. Corresponding methods and uses are also provided. The novel cell culture media and supplements are particularly beneficial when used in tissue engineering, for example during the in vitro cell culture of corneal cells and/or skin cells.
Claims18
Use of a serum-free or reduced-serum cell culture medium comprising a basal medium and one or more macromolecular crowding agents for in vitro cell culture to promote cell proliferation, wherein:
- a) the cell is a skin cell and the macromolecular crowding agent is selected from the group consisting of:
- PEG8, PEG35, PVP40, and PVP360;
- or a combination thereof;
- or
- b) the cell is a corneal cell and the macromolecular crowding agent is selected from the group consisting of:
- PEG8, PVP40, PVP360, and PEG35;
- or a combination thereof.
An in vitro serum-free or reduced-serum cell culture method to promote cell proliferation, comprising culturing cells in a serum-free or reduced-serum cell culture medium comprising a basal medium and one or more macromolecular crowding agents, wherein:
- a) the cells are skin cells and the macromolecular crowding agent is selected from the group consisting of:
- PEG8, PEG35, PVP40, PVP360;
- or a combination thereof;
- or
- b) the cells are corneal cells and the macromolecular crowding agent is selected from the group consisting of:
- PEG8, PVP40, PVP360, PEG35;
- or a combination thereof.
A serum-free or reduced-serum cell culture medium for in vitro cell culture, wherein the cell culture medium comprises a basal medium and one or more macromolecular crowding agents selected from the group consisting of:
- PEG8, PVP40, PVP360 and PEG35;
- or a combination thereof.
The cell culture medium, use or method according to any preceding claim, wherein the basal medium is DMEM/F12.
The cell culture medium, use or method according to any preceding claim, wherein the cell culture medium is a serum-free cell culture medium, optionally wherein the cell culture medium does not contain materials obtained from an animal.
The cell culture medium, use or method according to any preceding claim, wherein the cell culture medium further comprises glutamine, optionally wherein the cell culture medium further comprises ascorbic acid, insulin, transferrin, selenium, and ethanolamine.
The cell culture medium, use or method according to any preceding claim, wherein the cell culture medium further comprises penicillin and streptomycin.
A cell culture medium supplement for in vitro serum-free or reduced-serum cell culture, comprising one or more macromolecular crowding agents selected from the group consisting of:
- PVP40, PEG8, PVP360 and PEG35;
- or a combination thereof, wherein the supplement further comprises:
- insulin, transferrin, selenium, ethanolamine, ascorbic acid and/or glutamine.
A hermetically-sealed vessel containing a serum-free or reduced-serum cell culture medium or a cell culture medium supplement according to any of the preceding claims.
Dependencies are inferred from the claim text.