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OwnerCody Gilleland, Ph.D.inventor
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In the present invention the robotic microinjection platform construction is provided in which three cameras enable simultaneous video streaming of high magnification, low magnification camera views with a third horizon view for Z-depth information to enable microneedle alignment. The triple camera method in combination with image processing algorithms enables automated microneedle alignment and organism targeting in low magnification followed by microneedle manipulations of the organism in high magnification. The object detection algorithms are trained using neural networks on large image datasets. The object detection algorithms improve over time as the training dataset grows as data is acquired with more experimentation.
A method for delicately introducing genetic material into organisms by immobilizing, orienting and positioning organisms for precision microinjection and intracellular component extraction through a microneedle capillary with computer algorithm feedback from simultaneous low magnification and high magnification microscope with high-speed cameras.
A method for miniaturization and manufacturing of a digital microdosing platform employing surface mount electronics on a printed circuit board to enable multiplexed digital microdosing of scents and reagent chemicals using piezoelectric microblowers to produce a flow of air through a water and particle filter that is then routed by an array of piezoelectric bending actuators that actuate to lift and direct air flow through a swappable consumable array containing the individual scents between two one-way valves that prevent leakage while enabling scent production with spatiotemporal control enabled by cryptocurrency microtransactions via scent marketplace enabling real-time entertainment, gaming, advertisements, drug delivery, learning and memory enhancement and olfactory diagnostics for early viral testing, olfactory rehabilitation, neurodegeneration screening and prevention by stimulating olfactory system to enhance neural connections.
A method for rapid sex sorting of organisms by fluorescence detection using laser scanning at low intensity and then detecting fluorescence photon signal using a highly sensitive silicon photomultiplier device array coupled with the laser intensity control by amplifying transistor circuit to increase laser intensity to kill with nanosecond time resolution. The system can be set to select for fluorescent or non-fluorescent organisms and scan as a mesh conveyer belt moves organisms.
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A method of disrupting and extracting internal cellular components of a single cell in tissue using directed ultrasonic pressure waves, the method comprising:
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Dependencies are inferred from the claim text.