Lytic domain fusion constructs, checkpoint inhibitors, and methods of making and using same

A method of reducing or inhibiting proliferation of a cell, comprising contacting the cell with a fusion construct and a checkpoint inhibitor in an amount sufficient to reduce or inhibit proliferation of the cell, wherein the fusion construct comprises a first domain and a second domain, wherein the first domain consists of a 12 to 28 amino acid sequence that includes a peptide selected from KFAKFAKKFAKFAKK (SEQ. ID NO.1), KFAKFAKKFAKFAKKF (SEQ. ID NO.2), KFAKFAKKFAKFAKKFA (SEQ. ID NO.3), KFAKFAKKFAKFAKKFAK(SEQ. ID NO.4), KFAKFAKKFAKFAKKFAKF (SEQ. ID NO.5) and KFAKFAKKFAKFAKKFAKFA (SEQ. ID NO.6), or a 12 to 25 amino acid sequence that includes a peptide selected from KFAKFAKKFAKFAKK (SEQ. ID NO.1), KFAKFAKKFAKFAKKF (SEQ. ID NO.2), KFAKFAKKFAKFAKKFA (SEQ. ID NO.3), KFAKFAKKFAKFAKKFAK(SEQ. ID NO.4), KFAKFAKKFAKFAKKFAKF (SEQ. ID NO.5) and KFAKFAKKFAKFAKKFAKFA (SEQ. ID NO.6) having one or more of the K residues substituted with any of an For L residue, one or more of the F residues substituted with any of a K, A or L residue, or one or more of the A residues substituted with any of a K, For L residue;

• and the second domain comprises a binding moiety.