Method for extracting plasmid dna in bacteria

Abstract

Provided is a method for extracting plasmid DNA in bacteria, realizing lysis and neutralization during plasmid production in two mixing assemblies connected in series, and comprising the following steps: (1) mixing, (2) lysing, and (3) neutralizing. Step (1) is completed in a first mixing assembly; step (2) is completed in a lysis helical tube; step (3) is completed in a second mixing assembly; and the first mixing assembly, the lysis helical tube and the second mixing assembly are sequentially connected in series. A device used in the plasmid preparation process is simple, is convenient to operate, is low in costs, can remove a large amount of impurities during cell lysis without professional customized device and expensive device, has safe components, realizes automatic continuous lysis, and facilitates industrial production.

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