Using dried sodium hydroxide to denature double stranded dna
AssigneeIllumina, Inc.
InventorsEli Carrami, Oliver Miller, Sebastien Ricoult, Eilidh Rivers, Dale Weekes
Life of this patent
Estimating the term…
Abstract
This application relates to methods of denaturing double-stranded DNA (dsDNA). In some examples, the methods utilize dried sodium hydroxide. In some examples, the method includes loading dsDNA into a first portion of a cartridge, wherein the second portion of the cartridge contains sodium hydroxide in a dry form; and mixing the dsDNA with the sodium hydroxide, thereby denaturing the dsDNA.
Claims23
A method of denaturing double-stranded DNA (dsDNA), comprising:
- loading the dsDNA into a first portion of a cartridge, wherein sodium hydroxide is located in a second portion of the cartridge, wherein the sodium hydroxide is in a dry form, and
- wherein the dsDNA and the dried sodium hydroxide are not in contact with each other, when the dsDNA is loaded into the first portion of the cartridge;
- and
- mixing the dsDNA with the sodium hydroxide, thereby denaturing the dsDNA.
(canceled)
A method of denaturing double-stranded DNA (dsDNA), comprising:
- loading the dsDNA into an upper compartment of a spin tube that contains sodium hydroxide in a dry form, thereby denaturing the dsDNA,
- wherein a lower compartment of the spin tube contains a neutralizing agent;
- and
- centrifuging the spin tube to transfer the denatured DNA to the lower compartment of the spin tube, thereby neutralizing the sodium hydroxide.
(canceled)
A method of denaturing double-stranded DNA (dsDNA), comprising:
- loading dsDNA into a tube that contains sodium hydroxide in a dry form and a neutralizing agent comprising a time-triggered coat, thereby denaturing the dsDNA;
- and
- incubating the tube to allow for release of the neutralizing agent from the time-triggered coat, thereby neutralizing the sodium hydroxide.
23-64. (canceled)
Dependencies are inferred from the claim text.