US 2024/0218146 A1 · ENZYMES, MICRO-ORGANISMS AND USES THEREOF, AND A METHOD OF DEGRADING A POLYOLEFIN

Abstract

The present invention relates to the fields of life sciences, micro-organisms and degradation of polyolefin polymers. Specifically, the invention relates to an isolated specific enzyme or a fragment thereof, wherein said enzyme or fragment is capable of degrading a polyolefin, and to a micro-organism or a host cell comprising the enzyme or a fragment thereof. Also, the present invention relates to a polynucleotide encoding the enzyme or fragment thereof, and to an expression vector or plasmid comprising the polynucleotide of the present invention. And still, the present invention relates to use of the enzyme, fragment, micro-organism, host cell, polynucleotide, expression vector or plasmid of the present invention for degrading a polyolefin: to a method of degrading a polyolefin with the specific enzyme or a fragment thereof: and to a method of producing the enzyme or fragment thereof of the present invention.

Claims23

Dependent claims

A method of degrading a polyolefin, said method comprising

providing a material comprising a polyolefin and an enzyme or a fragment thereof capable of degrading the polyolefin, and
allowing said enzyme or fragment thereof to degrade the polyolefin,
wherein the enzyme or fragment thereof comprises a catalytic triad Ser-Asp-His corresponding to amino acids Ser97, Asp221 and His249 presented in SEQ ID NO:
2, and optionally one or more amino acids Gly99, Pro126 and Gly218 corresponding to the amino acids presented in SEQ ID NO:
2, and wherein the enzyme is optionally genetically modified.

2 more elements

An isolated enzyme or a fragment thereof comprising a catalytic triad Ser-Asp-His corresponding to amino acids Ser97, Asp221 and His249 presented in SEQ ID NO:

2, and optionally one or more amino acids Gly99, Pro126 and Gly218 corresponding to the amino acids presented in SEQ ID NO:
2, and wherein said enzyme or fragment is capable of degrading a polyolefin, wherein the enzyme is optionally genetically modified.

A micro-organism or a host cell comprising an enzyme or a fragment thereof comprising a catalytic triad Ser-Asp-His corresponding to amino acids Ser97, Asp221 and His249 presented in SEQ ID NO:

2, and optionally one or more amino acids Gly99, Pro126 and Gly218 corresponding to the amino acids presented in SEQ ID NO:
2, and wherein said enzyme or fragment is capable of degrading a polyolefin, wherein the enzyme is optionally genetically modified.

Dependencies are inferred from the claim text.

Enzymes, micro-organisms and uses thereof, and a method of degrading a polyolefin

Life of this patent

Enzymes, micro-organisms and uses thereof, and a method of degrading a polyolefin

Life of this patent

Abstract

Claims23