Compositions and methods for enhancing gene expression
AssigneeJanssen Pharmaceuticals, Inc.
InventorsKurt Iver KAMRUD, Maung Nyan WIN, Nathaniel Stephen WANG, Jason L. DEHART
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Abstract
The present disclosure generally relates to nucleic acid molecules for use in regulating gene expression. Disclosed herein include nucleic acid molecules containing one or more structural elements of the viral capsid enhancer operably linked to a coding sequence of a gene of interest. In some embodiments, the viral capsid enhancer comprises a Downstream Loop (DLP) from a viral capsid protein, or a variant of the DLP.
Claims43
A nucleic acid molecule, comprising a nucleic acid sequence encoding a modified viral RNA replicon, wherein the modified viral RNA replicon comprises:
- a first nucleic acid sequence encoding a viral capsid enhancer;
- and
- a second nucleic acid downstream from the first nucleic acid sequence, wherein the second nucleic acid sequence is operably linked to the first nucleic acid sequence, wherein the second nucleic acid sequence comprises a sequence encoding a nonstructural protein and a coding sequence for a gene of interest (GOI), wherein the modified viral RNA replicon is derived from an alphavirus or a virus species belonging to the Arterivirus genus of the Arteriviridae family;
- and the viral capsid enhancer is from an alpha virus.
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A RNA replicon comprising:
- a first nucleic acid sequence encoding a viral capsid enhancer;
- and
- a second nucleic acid downstream from the first nucleic acid sequence, wherein the second nucleic acid sequence is operably linked to the first nucleic acid sequence, wherein the second nucleic acid sequence comprises a sequence encoding a nonstructural protein and a coding sequence for a gene of interest (GOI), wherein the modified viral RNA replicon is derived from an alphavirus or a virus species belonging to the Arterivirus genus of the Arteriviridae family;
- and the viral capsid enhancer is from an alpha virus.
The replicon of claim 171, wherein the second nucleic acid sequence encodes nonstructural proteins nsp1-4.
Dependencies are inferred from the claim text.