Display substrate, manufacturing method thereof and display device

A display substrate, comprising:

• a base substrate;
• a pixel defining layer, disposed on the base substrate and including a plurality of openings;
• and
• at least one photo spacer, disposed on a side of the pixel defining layer away from the base substrate, wherein
• wherein the display substrate further comprises:
• a pixel circuit structure, disposed between the base substrate and the pixel defining layer and including a first signal line and a second signal line which are extended along a second direction,
• the pixel circuit structure also includes a power line, a first orthographic projection of the at least one photo spacer on the base substrate is partially overlapped with an orthographic projection of the power line on the base substrate.