US 2024/0203716 A1 · METHODS AND APPARATUS FOR MASS SPECTROMETRY

Abstract

Method and system of mass spectrometry is provided. The method comprises performing a first and a second MS1 scans on precursor ions produced by ionising a sample. The first MS1 scan comprises mass analysing a first set of the precursor ions transported to a mass analyser using an ion transport device operated at a first setting. The second MS1 scan comprises mass analysing a second set of the precursor ions transported to a mass analyser using the ion transport device operated at a second setting. Energies imparted on the precursor ions by the ion transport device are different in the first and second settings. Based on intensities of mass spectral peaks of the first and second MS1 scans, a mass spectral peak in at least one of the first MS1 scan or the second MS1 scan indicative of a precursor ion mass spectral peak is determined.

Claims31

Dependent claims

A method of mass spectrometry comprising:

ionising a sample to produce a plurality of precursor ions;
performing a first MS1 scan comprising:
transporting a first set of the precursor ions to a mass analyser using an ion transport device operated at a first setting;
and
mass analysing the first set of the precursor ions;
performing a second MS1 scan comprising:
transporting a second set of the precursor ions to a mass analyser using the ion transport device operated at a second setting;
and
mass analysing the second set of the precursor ions;
wherein the first setting and the second setting of the ion transport device are provided such that an energy imparted on the second set of precursor ions by the ion transport device is less than the energy imparted on the first set of precursor ions by the ion transport device;
and
determining if a mass spectral peak in at least one of the first MS1 scan or the second MS1scan is indicative of a precursor ion mass spectral peak based on relative intensities of mass spectral peaks of the first and second MS1 scans.

9 more elements

A mass spectrometer for analysing a sample comprising:

an ionisation source configured to ionise a sample to produce a plurality of precursor ions;
a mass analyser;
an ion transport device configured to transport precursor ions from the ionisation source to the mass analyser;
a controller configured:
(i) to perform a first MS1 scan by:
causing the ion transport device to transport a first set of the precursor ions to a mass analyser using a first setting of the ion transport device;
mass analysing the first set of the precursor ions using the mass analyser;
(ii) to perform a second MS1 scan by:
causing the ion transport device to transport a second set of the precursor ions to a mass analyser using a second setting of the ion transport device;
mass analysing the second set of the precursor ions using the mass analyser;
wherein the second setting of the ion transport device is configured to impart a lower amount of energy on the second set of precursor ions than an amount of energy imparted by the first setting of the ion transport device on the first set of precursor ions
(iii) to analyse a mass spectral peak in the first and/or second MS1 scan by determining if a mass spectral peak in at least one of the first or second MS1 scan is indicative of a precursor ion mass spectral peak based on relative intensities of the mass spectral peaks of the first and second MS1 scans.

9 more elements

Dependencies are inferred from the claim text.

Methods and apparatus for mass spectrometry

Life of this patent

Methods and apparatus for mass spectrometry

Life of this patent

Abstract

Claims31