Non-covalent halotag ligands

A non-covalently-HaloTag-binding compound characterized by the general formula (I)

• D-L-T (I), wherein
• a. D is or comprises a functional moiety Z selected from
• an organic dye moiety characterized by a molecular mass of between 300 g/mol and 1300 g/mol, particularly a fluorescent organic dye moiety;
• an affinity purification ligand,
• a pharmaceutical drug or a pharmaceutical drug candidate,
• an oligopeptide or a polypeptide,
• a nanoparticle,
• a solid surface or a matrix polymer,
• a nucleic acid oligomer,
• a carbohydrate,
• a lipid,
• a spectroscopic probe,
• a sensor, particularly a fluorescent sensor,
• a natural product, and
• a synthetic ligand binding to a biomolecule with an affinity of at least 100 μM,
• or
• b. D is a linkable moiety selected from
• an unprotected or protected amine moiety,
• a sulfonamide moiety,
• an N3 moiety,
• an alkyne moiety,
• a carboxylic acid or an activated form of a carboxylic acid, particularly an N-hydroxysuccinimide moiety,
• an ester moiety,
• an aldehyde,
• a thiol, and
• an isothiocyanate;
• L is a linear linker of 10-15 atoms in length, particularly wherein L is 10-11 atoms in length,
• wherein L comprises, particularly L essentially consists of, alkyl, trans-alkylene, and/or ether moieties and optionally one or several methyl substituents,
• particularly L is an unbranched linker that does not comprise methyl substituents;
• T is a moiety selected from the group comprising methylamine, monofluormethylamine, difluormethylamine, trifluormethylamine, methylsulfonamide, monofluormethylsulfonamide, difluormethylsulfonamide, trifluormethylsulfonamide, azide, acetamide, monofluoracetamide, difluoracetamide, and trifluoracetamide;
• particularly T is a moiety selected from the group comprising methylamine, trifluormethylsulfonamide and methylsulfonamide.