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US 2024/0201182 A1Published application
Inhibition of photon phenomena on single molecule arrays
AssigneeNautilus Subsidiary, Inc.
InventorsSeok Ki CHOI, Michael Augusto DARCY, Steven TAN, Pengyu HAO
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Abstract
Methods of providing photodamage inhibitors to single-analyte arrays are described. Photodamage inhibitors may be incorporated into various reagents that are subsequently coupled to single-analyte arrays. Photodamage inhibitors may be replenished during a single-analyte assay through provision of more reagents containing photodamage inhibitors. Useful reagent compositions containing photodamage inhibitors are described.
Claims20
A method, comprising:
- a) providing a single-analyte array comprising a plurality of sites, wherein individual sites of the plurality of sites each comprise one and only one macromolecule of a first plurality of macromolecules;
- b) binding a second plurality of macromolecules to macromolecules of the first plurality of macromolecules at a fraction of sites of the plurality of sites, thereby forming a macromolecule complex at respective sites of the fraction of sites, wherein the macromolecule complex comprises a macromolecule of the second plurality of macromolecules bound to one and only one macromolecule of the first plurality of macromolecules;
- c) detecting, in the presence of photons, a signal at each individual site of the first fraction of sites;
- and
- d) after detecting, in the presence of photons, the signal at respective sites of the first fraction of sites, dissociating macromolecules of the second plurality of macromolecules from macromolecules of the first plurality of macromolecules;
- wherein individual macromolecule complexes each comprise a plurality of photodamage inhibitor moieties that are coupled to the individual macromolecule complex.
3 more elements
An array composition, comprising:
- a) a plurality of sites, wherein individual sites of the plurality of sites each comprise one and only one macromolecule of a first plurality of macromolecules;
- b) at individual sites of a fraction of sites of the plurality of sites, a macromolecule of a second plurality of macromolecules bound to the one and only one macromolecule of the first plurality of macromolecules;
- and
- c) at individual sites of the fraction of sites of the plurality of sites, a plurality of photodamage inhibitor moieties coupled to a respective individual site;
- wherein the fraction of sites contains an average of at least 100 photodamage inhibitor moieties per site.
2 more elements
Dependencies are inferred from the claim text.