Method for detecting tcep content in adc by lc-ms/ms
AssigneeRemegen Co., Ltd.
InventorsXuejing YAO, Li WANG, Wenlong SUN, Guiping QI
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Abstract
The present disclosure relates to a method for detecting TCEP content in ADC by LC-MS/MS. The ADC samples are derivatized by Ellman reaction, and then the TCEP content is measured by LC-MS/MS. This method can exclude the interference caused by the substances that may react with Ellman in complex ADC samples, which provides with no need for sample pretreatment, and with strong specificity.
Claims12
A method for detecting TCEP content in ADC samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS), wherein the detected ADC samples are derivatized by Ellman reaction, and a C18 chromatographic column is used, without need for sample pretreatment.
Dependencies are inferred from the claim text.