Native Fluorescence Detection for Protein Analysis in Capillary Electrophoresis

Abstract

Methods and systems for determining concentration of a target protein in a sample using a capillary electrophoresis (CE) system are disclosed. In certain aspects, the method can include flowing a sample through a capillary tube of the CE system and utilizing a light source to generate radiation containing at least one excitation wavelength suitable for exciting at least one native fluorophore of at least one target protein in the sample. An excitation beam containing the at least one excitation wavelength can be directed onto a transparent portion of the capillary tube so as to excite said at least one native fluorophore of the target protein passing through a lumen of the transparent portion in order to cause the at least one native fluorophore to generate fluorescent radiation, and at least a portion of fluorescent radiation emitted by the excited target protein can be detected.

Dependencies are inferred from the claim text.