Primer set, reagent composition and method for the detection of methicillin-resistant staphylococcus aureus (mrsa)

A set of primers for amplifying the nucleotide sequence of the methicillin-resistant Staphylococcus aureus (MRSA) mecA gene, characterized in that it comprises a set of internal primers with the following nucleotide sequences a) and b), as well as a set of external primers comprising the following nucleotide sequences c) and d):

• a) 5′ GAAGGTGTGCTTACAAGTGCTAATA 3′ (SEQ ID NO:
• 3) or a sequence at least 90% identical to SEQ ID NO:
• 3, linked from the 3′ end, preferably by a TTTT bridge, to the sequence 5′ CAACATGAAAAATGATTATGGCTC 3′ (SEQ ID NO:
• 4) or a sequence at least 90% identical to SEQ ID NO:
• 4;
• b) 5′ TGACGTCTATCCATTTATGTATGGC 3′ (SEQ ID NO:
• 5) or a sequence at least 90% identical to SEQ ID NO:
• 5, linked from the 3′ end, preferably by a TTTT bridge, to the sequence 5′ AGGTTCTTTTTTTATCTTCGGTTA 3′ (SEQ ID NO:
• 6) or a sequence at least 90% identical to SEQ ID NO:
• 6;
• c) 5′ TGATGCTAAAGTTCAAAAGAGT 3′ (SEQ ID NO:
• 1) or a sequence at least 90% identical to SEQ ID NO:
• 1, and
• d) 5′ GTAATCTGGAACTTGTTGAGC 3′ nucleic sequence (SEQ ID NO:
• 2) or a sequence at least 90% identical to SEQ ID NO:
• 2.