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US 2024/0200135 A1Published application
Reusable Flow Cells Having Primer Binding Sites Comprising Reactive Sulfur Moieties and Methods of Using the Same, and Reagents for Use Therewith
AssigneeIllumina, Inc.
InventorsWeixian Xi, Brian Mather, Claudia Battistella
Life of this patent
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Abstract
Reusable flow cells for sequencing which exhibit signal intensity retention over numerous use cycles, the active surface of which contains reactive sulfur moieties for reversible primer binding, methods of using such flow cells, reagents therefor, and kits containing the same.
Claims20
A flow cell comprising a substrate, the substrate comprising a plurality of primer binding sites, each of the plurality of primer binding sites comprising a reactive sulfur moiety.
A method comprising:
- providing a flow cell having a substrate comprising a plurality of primer binding sites, each of
- the plurality of primer binding sites comprising a first reactive sulfur moiety;
- grafting oligonucleotide primers to a portion of the plurality of primer binding sites;
- performing a nucleic acid sequence analysis;
- and
- contacting the flow cell substrate with a cleaving reagent that removes the oligonucleotide primers such that the substrate comprises a plurality of primer binding sites comprising a second reactive sulfur moiety.
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A kit comprising:
- a flow cell having a substrate comprising a plurality of primer binding sites, each of the plurality of primer binding sites comprising a first reactive sulfur moiety;
- a priming reagent comprising oligonucleotide primers having a terminal group that forms a bond with the primer binding sites;
- and
- a cleaving reagent that is capable of removing the oligonucleotide primers such that the substrate comprises a plurality of primer binding sites comprising a second reactive sulfur moiety.
1 more element
Dependencies are inferred from the claim text.