Multi-Metabolite Monitoring Biosensor

A biosensor for monitoring one or more metabolites within a base fluid comprising:

• one or more electrodes comprising:
• a working electrode having a plurality of layers including:
• a catalyst layer deposited over the inert conductive layer comprising a nanocomposite through which hydrogen peroxide may be adsorbed;
• and
• an enzyme layer deposited over the catalyst layer comprising one or more enzymes selected to perform a redox reaction towards one or more metabolites;
• and
• an electrical interface in electrical communication with the one or more electrodes configured to receive an electrical signal resulting from a biochemical reaction at or generated by one or more of the layers, the electrical signal corresponding to a level of the one or more metabolites in the base fluid.

A biosensor for monitoring one or more metabolites within a base fluid comprising:

• a plurality of electrodes comprising:
• a first working electrode having a plurality of layers including:
• a catalyst layer deposited over the inert conductive layer comprising a nanocomposite through which hydrogen peroxide may be adsorbed;
• and
• an enzyme layer deposited over the catalyst layer comprising one or more enzymes selected to perform a redox reaction towards a first metabolite;
• a second working electrode having a plurality of layers including:
• a catalyst layer deposited over the inert conductive layer comprising a nanocomposite through which hydrogen peroxide may be adsorbed;
• and
• an enzyme layer deposited over the catalyst layer comprising one or more enzymes selected to perform a redox reaction towards a second metabolite different than the first metabolite;
• and
• an electrical interface in electrical communication with the one or more electrodes configured to receive an electrical signal resulting from a biochemical reaction at or generated by one or more of the layers, the electrical signal corresponding to a level of the one or more metabolites in the base fluid.