Packaging cells with targeted gene knockouts that improve retroviral vector titers
AssigneeThe Regents of the University of California
InventorsDonald B. Kohn, Roger Paul Hollis, Jiaying Han
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Abstract
In various embodiments packaging cells are provided that increase provide increased titer and are useful for lentiviral production. In certain embodiments the packaging cells comprise a knockout of one or more genes selected from the group consisting of IFNAR1, ATR, OAS1, LDLR, and PKR.
Claims54
A recombinant retroviral packaging cell, said packaging cell comprising:
- a mammalian cell wherein one or more mammalian genes that inhibit virus production are knocked out or knocked down, said one or more mammalian genes that inhibit virus production comprise
- (a) a mammalian gene that encodes a low-density lipoprotein receptor (LDLR);
- (b) a mammalian gene that encodes interferon alpha and beta receptor subunit 1 (IFNAR1) or interferon alpha and beta receptor subunit 2 (IFNAR2) or a combination thereof of;
- (c) one or more mammalian genes that regulates the DNA damage response pathway;
- (d) one or more mammalian genes that regulates transcription;
- or
- (e) one or more mammalian genes that regulates innate immunity;
- or
- (f) any combination of (a)-(e) above.
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Dependencies are inferred from the claim text.