Use of protoporphyrinogen oxidase

A planting combination for controlling the growth of weeds, comprising a PPO-inhibitor herbicide and at least one transgenic plant, wherein the herbicide containing an effective dose of a PPO inhibitor is applied to a field where the at least one transgenic plant is present, wherein the transgenic plant comprises in its genome a polynucleotide sequence encoding a protoporphyrinogen oxidase, and the transgenic plant has a reduced plant damage and/or increased plant yield compared with other plants without the polynucleotide sequence encoding the protoporphyrinogen oxidase;

• wherein the protoporphyrinogen oxidase has at least 88% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• preferably, the protoporphyrinogen oxidase has at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• preferably, the protoporphyrinogen oxidase has at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• more preferably, the protoporphyrinogen oxidase has at least 99% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• further preferably, the protoporphyrinogen oxidase is selected from the amino acid sequences of the group consisting of SEQ ID NOs:1-14;
• preferably, the transgenic plant comprises monocotyledonous plants and dicotyledonous plants;
• more preferably, the transgenic plant is oats, wheat, barley, millet, corn, sorghum, Brachypodium distachyon, rice, tobacco, sunflower, alfalfa, soybean, Cicer arietinum, peanut, sugar beet, cucumber, cotton, oilseed rape, potato, tomato or Arabidopsis thaliana;
• further preferably, the transgenic plant is a glyphosate-tolerant plant, and the weeds are glyphosate-resistant weeds,
• preferably, the PPO-inhibitor herbicide comprises a PPO-inhibitor herbicide from the class of diphenyl ethers, oxadiazolones, N-phenylphthalimides, oxazolinones, phenylpyrazoles, uracils, thiadiazoles, triazolinones and/or triazinones;
• further preferably, the PPO-inhibitor herbicide comprises oxyfluorfen, saflufenacil, sulfentrazone, and/or flumioxazin.

A method for generating a plant which is tolerant to a PPO-inhibitor herbicide, characterized in that the method comprises introducing a polynucleotide sequence encoding a protoporphyrinogen oxidase into the genome of the plant, and when the herbicide containing an effective dose of a PPO inhibitor is applied to a field where at least the plant is present, the plant has a reduced plant damage and/or increased plant yield compared with other plants without the polynucleotide sequence encoding the protoporphyrinogen oxidase, wherein the protoporphyrinogen oxidase has at least 88% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;

• preferably, the protoporphyrinogen oxidase has at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• preferably, the protoporphyrinogen oxidase has at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• more preferably, the protoporphyrinogen oxidase has at least 99% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• further preferably, the protoporphyrinogen oxidase is selected from the amino acid sequences of the group consisting of SEQ ID NOs:1-14;
• preferably, the introduction method comprises genetic transformation, genome editing or gene mutation methods;
• preferably, the plant comprises monocotyledonous plants and dicotyledonous plants;
• more preferably, the plant is oats, wheat, barley, millet, corn, sorghum, Brachypodium distachyon, rice, tobacco, sunflower, alfalfa, soybean, Cicer arietinum, peanut, sugar beet, cucumber, cotton, oilseed rape, potato, tomato or Arabidopsis thaliana;
• preferably, the PPO-inhibitor herbicide comprises a PPO-inhibitor herbicide from the class of diphenyl ethers, oxadiazolones, N-phenylphthalimides, oxazolinones, phenylpyrazoles, uracils, thiadiazoles, triazolinones and/or triazinones;
• further preferably, the PPO-inhibitor herbicide comprises oxyfluorfen, saflufenacil, sulfentrazone, and/or flumioxazin.

A method for cultivating a plant which is tolerant to a PPO-inhibitor herbicide, characterized by comprising:

• planting at least one plant propagule, wherein the plant propagule comprises in its genome a polynucleotide encoding a protoporphyrinogen oxidase, wherein the protoporphyrinogen oxidase has at least 88% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• allowing the plant propagule to grow into a plant;
• and
• applying the herbicide comprising an effective dose of a PPO inhibitor to a field comprising at least the plant, and harvesting the plant having a reduced plant damage and/or increased plant yield compared with other plants without the polynucleotide sequence encoding the protoporphyrinogen oxidase;
• preferably, the protoporphyrinogen oxidase has at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• preferably, the protoporphyrinogen oxidase has at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• more preferably, the protoporphyrinogen oxidase has at least 99% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• further preferably, the protoporphyrinogen oxidase is selected from the amino acid sequences of the group consisting of SEQ ID NOs:1-14;
• preferably, the plant comprises monocotyledonous plants and dicotyledonous plants;
• more preferably, the plant is oats, wheat, barley, millet, corn, sorghum, Brachypodium distachyon, rice, tobacco, sunflower, alfalfa, soybean, Cicer arietinum, peanut, sugar beet, cucumber, cotton, oilseed rape, potato, tomato or Arabidopsis thaliana;
• preferably, the PPO-inhibitor herbicide comprises a PPO-inhibitor herbicide from the class of diphenyl ethers, oxadiazolones, N-phenylphthalimides, oxazolinones, phenylpyrazoles, uracils, thiadiazoles, triazolinones and/or triazinones;
• further preferably, the PPO-inhibitor herbicide comprises oxyfluorfen, saflufenacil, sulfentrazone, and/or flumioxazin.

A method for protecting a plant from damages caused by a PPO-inhibitor herbicide or for conferring tolerance to the PPO-inhibitor herbicide upon a plant, characterized by comprising applying the herbicide comprising an effective dose of a PPO inhibitor to a field where at least one transgenic plant is present, wherein the transgenic plant comprises in its genome a polynucleotide sequence encoding a protoporphyrinogen oxidase, and the transgenic plant has a reduced plant damage and/or increased plant yield compared with other plants without the polynucleotide sequence encoding the protoporphyrinogen oxidase, wherein the protoporphyrinogen oxidase has at least 88% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;

• preferably, the protoporphyrinogen oxidase has at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• preferably, the protoporphyrinogen oxidase has at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• more preferably, the protoporphyrinogen oxidase has at least 99% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• further preferably, the protoporphyrinogen oxidase is selected from the amino acid sequences of the group consisting of SEQ ID NOs:1-14;
• preferably, the transgenic plant comprises monocotyledonous plants and dicotyledonous plants;
• more preferably, the transgenic plant is oats, wheat, barley, millet, corn, sorghum, Brachypodium distachyon, rice, tobacco, sunflower, alfalfa, soybean, Cicer arietinum, peanut, sugar beet, cucumber, cotton, oilseed rape, potato, tomato or Arabidopsis thaliana;
• preferably, the PPO-inhibitor herbicide comprises a PPO-inhibitor herbicide from the class of diphenyl ethers, oxadiazolones, N-phenylphthalimides, oxazolinones, phenylpyrazoles, uracils, thiadiazoles, triazolinones and/or triazinones;
• further preferably, the PPO-inhibitor herbicide comprises oxyfluorfen, saflufenacil, sulfentrazone, and/or flumioxazin.

Use of a protoporphyrinogen oxidase for conferring tolerance to a PPO-inhibitor herbicide upon a plant, wherein the protoporphyrinogen oxidase has at least 88% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;

• preferably, the protoporphyrinogen oxidase has at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• preferably, the protoporphyrinogen oxidase has at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• more preferably, the protoporphyrinogen oxidase has at least 99% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:1-14;
• further preferably, the protoporphyrinogen oxidase is selected from the amino acid sequences of the group consisting of SEQ ID NOs:1-14;
• preferably, the use of the protoporphyrinogen oxidase for conferring tolerance to a PPO-inhibitor herbicide upon a plant comprises applying the herbicide containing an effective dose of a PPO inhibitor to a field where at least one transgenic plant is present, wherein the transgenic plant comprises in its genome a polynucleotide sequence encoding the protoporphyrinogen oxidase, and the transgenic plant has a reduced plant damage and/or increased plant yield compared with other plants without the polynucleotide sequence encoding the protoporphyrinogen oxidase;
• preferably, the plant comprises monocotyledonous plants and dicotyledonous plants;
• more preferably, the plant is oats, wheat, barley, millet, corn, sorghum, Brachypodium distachyon, rice, tobacco, sunflower, alfalfa, soybean, Cicer arietinum, peanut, sugar beet, cucumber, cotton, oilseed rape, potato, tomato or Arabidopsis thaliana;
• preferably, the PPO-inhibitor herbicide comprises a PPO-inhibitor herbicide from the class of diphenyl ethers, oxadiazolones, N-phenylphthalimides, oxazolinones, phenylpyrazoles, uracils, thiadiazoles, triazolinones and/or triazinones;
• further preferably, the PPO-inhibitor herbicide comprises oxyfluorfen, saflufenacil, sulfentrazone, and/or flumioxazin.