Method for Constructing RNA Sequencing Library, Sequencing Method and Kit

Abstract

Provided in the present application is a method for constructing an RNA sequencing library, a sequencing method and a kit. The construction method comprises: acquiring a single-stranded cDNA, which is a reverse transcription product of mRNA, the 3′-terminal of the single-stranded cDNA including a cDNA tag sequence; cyclizing the single-stranded cDNA to obtain a single-stranded cyclized cDNA; amplifying the single-stranded cyclized cDNA with a primer combination, which is formed by a random primer or a gene-specific primer and a cDNA tag primer, so as to obtain an amplified fragment, the cDNA tag primer being at least a part of the cDNA tag sequence; and performing fragmentation for library construction on the amplified fragment, so as to obtain an RNA sequencing library.

Dependencies are inferred from the claim text.