Lysis buffers for extracting nucleic acids
AssigneeLife Technologies Corporation
InventorsJames STRAY, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
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Abstract
The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.
Claims34
-67. (canceled)
A method of identification comprising:
- (a) lysing a sample in a lysis solution, forming a lysate comprising a nucleic acid from the sample;
- (b) extracting the lysate, wherein the nucleic acid is in the lysate;
- (c) analyzing the nucleic acid;
- and
- (d) identifying the sample based on the nucleic acid analysis.
69) The method according to claim 76, wherein the sample is a fingerprint tape lift.
70) The method of claim 51, wherein the lysis solution comprises one or more of a detergent, a chelating agent, a reducing agent, and an enzyme.
71) The method of claim 59, wherein the detergent is selected from one or more of N-lauroyl sarcosine, sodium deoxycholate, CTAB, dodecyl β-D-maltoside, nonanoyl-N-methylglucamide, polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether, sodium dodecyl sulfate, and combinations thereof.
72) The method of claim 59, wherein the chelating agent comprises at least one of ethylene glycol tetraacetic acid (EGTA), ethylene diamine tetraacetic acid (EDTA) and citric acid.
73) The method of claim 59, wherein the reducing agent comprises at least one of tris(2-carboxyethyl)phosphine (TCEP) dithioerythritol (DTE), and dithiothreitol (DTT).
74) The method of claim 59, wherein the enzyme comprises one or more of caspase, chymotrypsin, pepsin, proteinase K, thrombin, Staphylococcus V8 protease, pronase, papain, Bacillus sp. E1A protease, and trypsin and combinations thereof.
76) A kit for extracting nucleic acid from a solid sample comprising:
- a lysis buffer.
93) The composition of claim 1, further comprising a salt selected from KCl, NaCl, MgCl, MnCl2.
94) A composition for lysing a biological sample on denim comprising one or more of one or more of a detergent, a chelating agent, a salt, a reducing agent, and an enzyme.
Dependencies are inferred from the claim text.