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AssigneesPrecision BioSciences, Inc., University of Miami
InventorsCarlos T. Moraes, James Jefferson Smith, Derek Jantz, Ginger H. Tomberlin, John Morris, Wendy Shoop
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Disclosed herein are recombinant meganucleases engineered to recognize and cleave a recognition sequence present in the human mitochondrial DNA (mtDNA). The disclosure further relates to the use of such recombinant meganucleases in combination with mitochondrial transit peptides in methods for producing genetically-modified eukaryotic cells, and to a population of genetically-modified eukaryotic cells wherein the mtDNA has been modified or edited.
A mitochondria-targeting engineered meganuclease (MTEM) that binds and cleaves a recognition sequence in mitochondrial genomes of a eukaryotic cell, wherein said MTEM comprises an engineered meganuclease attached to a mitochondrial transit peptide (MTP).
Dependencies are inferred from the claim text.