Nuclease with improved targeting activity

Abstract

The present invention relates to a polynucleotide encoding a nuclear polypeptide comprising a cargo polypeptide and an N-terminal activity-optimizing peptide (NAO peptide), wherein said NAO peptide comprises (i) a tag peptide, (ii) a linker peptide; and (iii) a nuclear localization sequence (NLS) peptide wherein said linker peptide comprises at least three small amino acids independently selected from glycine, alanine, leucine, serine, aspartate, asparagine, threonine, phenylalanine, glutamate, glutamine, histidine, arginine, lysine, valine, isoleucine, and proline, more preferably from glycine, alanine, proline, serine, valine, and threonine; and wherein said tag peptide is fused at its C-terminus to the linker peptide; and to vectors, polypeptides, host cells, and methods related thereto.

Dependencies are inferred from the claim text.