Modified Pyrococcus Polymerases and Uses Thereof

A polymerase comprising an amino acid sequence that is at least 80% identical to a continuous 500 amino acid sequence within SEQ ID NO:

• 1;
• comprising the following amino acids:
• a valine at amino acid position 486 or at an amino acid position functionally equivalent to amino acid position 486;
• a serine at amino acid position 515 or at an amino acid position functionally equivalent to amino acid position 515;
• and
• at least one of the following amino acids:
• an alanine at amino acid position 215 or at an amino acid position functionally equivalent to amino acid position 215;
• an alanine at amino acid position 315 or at an amino acid position functionally equivalent to amino acid position 315;
• an alanine at amino acid position 477 or at an amino acid position functionally equivalent to amino acid position 477;
• a tryptophan at amino acid position 477 or at an amino acid position functionally equivalent to amino acid position 477;
• an alanine at amino acid position 478 or at an amino acid position functionally equivalent to amino acid position 478;
• an alanine at amino acid position 603 or at an amino acid position functionally equivalent to amino acid position 603;
• an alanine at amino acid position 714 or at an amino acid position functionally equivalent to amino acid position 714;
• an alanine at amino acid position 719 or at an amino acid position functionally equivalent to amino acid position 719;
• or
• an alanine at amino acid position 736 or at an amino acid position functionally equivalent to amino acid position 736.