Method for producing virus particles

Abstract

It is an object of the present invention to provide a method for producing a virus to obtain high-purity virus particles, said method being more efficient than conventional methods. Specifically, the present invention relates to a method for producing full-genome virus particles, comprising steps of purifying full-genome virus particles from a virus particle mixed solution containing empty virus particles, intermediate virus particles and the full-genome virus particles, wherein the method comprises: a step (a) of rotating a zonal rotator at a low speed and arranging the virus particle mixed solution, a liquid having a lower density than the full-genome virus particles (liquid L), and a liquid having a higher density than the liquid L (liquid H1) in this order from the rotation axis side of the rotor toward the outside; a step (b) of operating the zonal rotor after the step (a) at an ultracentrifugation mode, so as to separate the empty virus particles, the intermediate virus particles and the full-genome virus particles from one another; and a step (c) of removing the contents of the zonal rotor after the step (b), while fractionating the contents, so as to recover a fraction comprising the full-genome virus particles.

Dependencies are inferred from the claim text.