Method of making in vivo human small intestine organoids from pluripotent stem cells
AssigneeChildren's Hospital Medical Center
InventorsJames M. Wells, Carey Lane Watson, Jorge Orlando Munera, Maxime Mickael Mahe, Michael A. Helmrath, Michael J. Workman
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Abstract
Disclosed are methods for making a vascularized hollow organ derived from human intestinal organoid (HIOs). The HIOs may be obtained from human embryonic stem cells (ESC's) and/or induced pluripotent stem cells (iPSCs), such that the HIO forms mature intestinal tissue. Also disclosed are methods for making a human intestinal tissue containing a functional enteric nervous system (ENS).
Claims16
A method of making a vascularized hollow organ comprising the steps of
- a) engrafting a human intestinal organoid (HIO) into an immune compromised mammal;
- wherein said HIO is obtained from human embryonic stem cells (ESC's) and/or induced pluripotent stem cells (iPSCs);
- wherein, during said engrafting step, said HIO forms mature intestinal tissue.
A method of making a human intestinal tissue containing a functional enteric nervous system (ENS) comprising the steps of
- a) contacting vagal-like neural crest cells (NCCs) derived from human ES cells and/or iPS cells (IPCs) with a three dimensional human intestinal organoid (HIO);
- and
- c) transplanting said HIO in vivo.
Dependencies are inferred from the claim text.