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US 2024/0200032 A1Published application
Method for differentiation to stem cell derived hyaline cartilage spheroid and uses thereof
AssigneeCelloid Co., Ltd.
InventorsDong Sung KIM, Ji Hyeon JU, Yoo Jun NAM, Yeri RIM, Seong Jin LEE, Do Hui KIM
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Abstract
The present invention relates to a culture method for culturing three-dimensional cell aggregates, and a culture device therefor, and more particularly, to a method and an device for differentiating stem cells in aggregate form into hyaline chondrocyte aggregates, and the use of differentiated hyaline chondrocyte aggregates.
Claims12
A method of differentiating stem cells into hyaline a hyaline chondrocyte aggregate, the method comprising steps of:
- a) preparing a stem cell aggregate by aggregating stem cells;
- b) culturing the stem cell aggregate, prepared in step a), for 1 to 10 days under hypoxic conditions corresponding to an oxygen level of 0.001 to 0.15 mol/m3;
- and
- c) differentiating the cell aggregate, cultured in step b), into a hyaline chondrocyte aggregate in a permeable well or a permeable tube.
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Dependencies are inferred from the claim text.