Culture medium, coating matrix and method for maturing midbrain dopaminergic progenitor cells
AssigneeNuwacell Biotechnologies Co., Ltd.
InventorsJunying YU, Luyan JIAO, Yongqiang ZHAO, Ying ZHANG
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Abstract
The present disclosure described herein provides, inter alia, an expansion method for expanding mDAPs and a culture medium and a coating matrix combination used in the expansion method, as well as a maturation method for maturing mDAPs and a culture medium used in the maturation method. The present disclosure also provides a substantially homogeneous population of mDAPs and a substantially homogeneous population of mDANs.
Claims31
A culture medium that is capable of promoting maturation of mDAPs (midbrain Dopaminergic Progenitor cells), said culture medium comprising:
- (a) a neural basal medium;
- (b) a heat treated human Platelet Lysate (HhPLT);
- (c) a Transforming Growth Factor β (TGF-β);
- (d) a γ-secretase inhibitor;
- and
- (e) a cAMP-based compound or its cyclase activator.
(canceled)
A method for promoting the maturation of midbrain Dopaminergic Progenitor cells (mDAPs), comprising contacting the mDAPs with a ROCK inhibitor-containing maturation medium on a culture surface coated with a coating matrix combination comprising:
- (a) a first coating matrix that can support cell adhesion for the mDAPs, wherein the first coating matrix is not laminin;
- and, (b) a second coating matrix that can improve the maturity for the mDAPs, wherein the second coating matrix comprises a polylysine-based compound and/or a polyornithine-based compound.
Dependencies are inferred from the claim text.