Culture medium, coating matrix and method for expanding midbrain dopaminergic progenitor cells
AssigneeNuwacell Biotechnologies Co., Ltd.
InventorsJunying YU, Luyan JIAO, Yongqiang ZHAO, Ying ZHANG
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Abstract
The present disclosure described herein provides, inter alia, an expansion method for expanding mDAPs and a culture medium and a coating matrix combination used in the expansion method, as well as a maturation method for maturing mDAPs and a culture medium used in the maturation method. The present disclosure also provides a substantially homogeneous population of mDAPs and a substantially homogeneous population of mDANs.
Claims24
1-17. (canceled)
A culture system comprising midbrain Dopaminergic Progenitor cells (mDAPs) and a cell culture substrate coated with a coating matrix combination that promotes the expansion of the mDAPs, wherein said coating matrix combination comprises:
- (a) a first coating matrix that supports cell adhesion for the mDAPs, wherein the first coating matrix is selected from the group consisting of vitronectin (VTN), collagen, proteoglycan, fibronectin, entactin, elastin, laminin, a functional fragment of any preceding protein, hyaluronic acid, gelatin, and a combination thereof;
- and,
- (b) a second coating matrix that improves the expression of mDAP-specific markers during the expansion and passaging of the mDAPs as compared to that in the absence of the second coating matrix, wherein the second coating matrix comprises a Notch agonist.
(canceled)
23-47. (canceled)
Dependencies are inferred from the claim text.