Compositions for preserving anaerobic microorganisms and methods of making and using the same
OwnerAaron Cowleyinventor
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Abstract
Provided herein are compositions for preserving one or more species of anaerobic microorganisms. Also provided are preserved compositions of anaerobic microorganisms, e.g., cryopreserved compositions, as well as methods for preparing and using the preserved compositions.
Claims62
A composition for preserving anaerobic microorganisms, the composition comprising:
- a viscosity modifier, and an energy source for the microorganisms, and, when solvated in an aqueous solvent, has a viscosity in the range of about 5 cP to about 6,000 cP, an osmolality in the range of about 300 Osm/L to about 4,000 Osm/L, a pH in the range of about 4 to about 8, and optionally a redox potential in the range of −50 mV to −500 mV.
A cryopreservative composition for cryopreserving anaerobic microorganisms, the composition comprising:
- a viscosity modifier, an energy source for the microorganisms, and, when solvated in an aqueous solvent, has a viscosity in the range of about 5 cP to about 6,000 cP, an osmolality in the range of about 300 Osm/L to about 4,000 Osm/L, a pH in the range of about 4 to about 8, and optionally a redox potential in the range of −50 mV to −500 mV.
A cryopreservative composition for cryopreserving anaerobic microorganisms, the composition comprising:
- (i) about 30% (w/w) to about 50% (w/w) of a cell paste, wherein the cell paste comprises one or more anaerobic microorganisms;
- (ii) about 3% (w/w) to about 15% (w/w) of an energy source for the microorganisms;
- (iii) about 0.01% (w/w) to about 0.4% (w/w) of a viscosity modifier;
- and
- (iv) about 30% (w/w) to about 60% (w/w) of an aqueous solvent,
- wherein the cryopreservative composition has a viscosity in the range of about 5 cP to about 6,000 cP, an osmolality in the range of about 300 Osm/L to about 4,000 Osm/L, and a pH in the range of about 4 to about 8.
A cryopreservative composition for cryopreserving anaerobic microorganisms, the composition comprising:
- (i) about 30% (w/w) to about 50% (w/w) of a cell paste, wherein the cell paste comprises one or more anaerobic microorganisms;
- (ii) about 0.5% (w/w) to about 5% (w/w) raftilose;
- (iii) about 3% (w/w) to about 10% (w/w) maltodextrin;
- (iv) about 0.01% (w/w) to about 0.4% (w/w) sodium alginate;
- (v) about 5% (w/w) to about 10% (w/w) trehalose;
- (vi) 0% (w/w) to about 5% (w/w) sucrose;
- and
- (vii) about 35% (w/w) to about 55% (w/w) water,
- wherein the cryopreservative composition has a viscosity in the range of about 5 cP to about 6,000 cP, an osmolality in the range of about 300 Osm/L to about 4,000 Osm/L, and a pH in the range of about 4 to about 8.
A preserved composition comprising:
- one or more anaerobic microorganisms, a viscosity modifier, an energy source for the microorganism, a redox potential in the range of −50 mV to −500 mV, and a water content of less than 5% (w/w).
A cryopreserved composition comprising:
- one or more anaerobic microorganisms, a viscosity modifier, an energy source for the microorganism, a redox potential in the range of −50 mV to −500 mV, and a water content of less than 5% (w/w).
A cryopreserved composition comprising:
- (i) one or more anaerobic microorganisms;
- (ii) an energy source comprising raftilose and maltodextrin;
- (iii) sodium alginate;
- (iv) a protein stabilizer comprising trehalose, sucrose, and combinations thereof and (vi) a water content of less than 5% (w/w).
Dependencies are inferred from the claim text.