Mutant lysenin pores
AssigneeOxford Nanopore Technologies PLC
InventorsMark John Bruce, James Anthony Clarke, Andrew John Heron, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace
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Abstract
The invention relates to mutant forms of lysenin. The invention also relates to analyte characterisation using lysenin.
Claims22
-32. (canceled)
A method of characterising a target analyte, comprising:
- (a) contacting the target analyte with a protein pore such that the target analyte moves through the protein pore, wherein the pore comprises at least one lysenin monomer that comprises a barrel-forming region comprising the amino acid sequence as set forth in positions 44 to 126 of SEQ ID NO:
- 2 with one or more amino acid substitutions, wherein at least one of the amino acid substitutions introduces a cysteine, lysine, or non-natural amino acid;
- and
- (b) taking one or more measurements as the target analyte moves through the protein pore, wherein the measurements are indicative of one or more characteristics of the target analyte, thereby characterising the target analyte.
-50. (canceled)
The method according to claim 38, wherein the method comprises characterising two or more target analytes, wherein (a) comprises contacting the two or more target analytes-with the protein pore such that each of the two or more target analytes move through the protein pore and (b) comprises taking measurements as the two or more target analytes move through the protein pore, wherein the measurements are indicative of one or more characteristics of each of the two or more target analytes.
Dependencies are inferred from the claim text.