Chimeric newcastle disease virus expressing apmv hn and f proteins

A nucleic acid sequence comprising:

• (1) a transcription unit encoding a NDV nucleocapsid (N) protein, (2) a transcription unit encoding a NDV phosphoprotein (P), (3) a transcription unit encoding a NDV matrix (M) protein, (4) a transcription unit encoding a NDV large polymerase (L), and (5) the nucleotide sequence of any one of SEQ ID NOS:1-14, or a nucleotide sequence that is at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% identical to the nucleotide sequence of any one of SEQ ID NOS:1-14.

A nucleic acid sequence comprising the nucleotide sequence of SEQ ID NO:44, or SEQ ID NO:44 without the GFP coding sequence.

A nucleic acid sequence comprising a nucleotide sequence that is at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% identical to the nucleotide sequence of SEQ ID NO:44, or SEQ ID NO:44 without the GFP coding sequence.

A nucleic acid sequence comprising the nucleotide sequence of SEQ ID NO:45, or SEQ ID NO:45 without the GFP coding sequence.

A nucleic acid sequence comprising a nucleotide sequence that is at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% identical to the nucleotide sequence of SEQ ID NO:45, or SEQ ID NO:45 without the GFP coding sequence.

A recombinant Newcastle disease virus (NDV) comprising a packaged genome, wherein the packaged genome comprises a nucleotide sequence of a Newcastle disease virus genome in which (1) the nucleotide sequence encoding the NDV HN protein has been replaced with a nucleotide sequence encoding a non-NDV APMV HN protein, wherein NDV intergenic regions are before and after the non-NDV AMPV HN protein coding sequence;

• and (2) the nucleotide sequence encoding the NDV F protein has been replaced with a nucleotide sequence encoding a non-NDV APMV F protein, wherein NDV intergenic regions are before and after the non-NDV AMPV F protein coding sequence, and wherein the non-NDV APMV HN protein and non-NDV APMV F protein are not NDV HN protein and F proteins, respectively.

A recombinant Newcastle disease virus (NDV) comprising a packaged genome, wherein the packaged genome comprises a nucleotide sequence of a Newcastle disease virus genome in which the nucleotide sequences encoding the NDV HN protein and NDV F protein are replaced with a nucleotide sequence comprising a negative sense RNA sequence transcribed from the cDNA sequence set forth in any one of SEQ ID NOs:1-14.

A recombinant Newcastle disease virus (NDV) comprising a packaged genome, wherein the packaged genome comprises a nucleotide sequence of a Newcastle disease virus genome in which the nucleotide sequences encoding the NDV HN protein and NDV F protein are replaced with a nucleotide sequence comprising a negative sense RNA sequence transcribed from a nucleotide sequence that is at least 80%, at least 85%, at least 90%, at least 95%, or at least 98% identical to the nucleotide sequence of any one of SEQ ID NOS:1-14.