Antigen recognizing receptors targeting cd33 and uses thereof

An antigen-recognizing receptor, comprising an extracellular antigen-binding domain, a transmembrane domain, and an intracellular signaling domain, wherein the extracellular antigen-binding domain specifically binds to CD33, wherein the extracellular antigen-binding domain comprises a heavy chain variable region and a light chain variable region, wherein:

• (a) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 2, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 3, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 4;
• the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 5, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 6, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 7;
• (b) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 12, a CDR2 comprising an amino acid sequence set forth in SEQ ID NO:
• 13, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 14;
• the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 15, and a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 16, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 17;
• (c) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 22, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 23, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 24 the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 25, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 26, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 27;
• (d) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 32, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 33, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 34 the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 25, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 26, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 27;
• (e) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 37, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 38, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 39;
• the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 40, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 41, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 42;
• (f) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 47, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 38, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 48;
• the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 49, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 50, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 51;
• (g) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 56, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 57, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 58;
• the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 59, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 60, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 61;
• (h) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 66, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 67, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 68;
• the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 69, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 70, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 71;
• (i) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 76, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 57, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 77;
• the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 79, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 80;
• or
• (j) the heavy chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 85, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 86, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 87;
• the light chain variable region comprises a CDR1 comprising the amino acid sequence set forth in SEQ ID NO:
• 5, a CDR2 comprising the amino acid sequence set forth in SEQ ID NO:
• 41, and a CDR3 comprising the amino acid sequence set forth in SEQ ID NO:
• 42.