Modified tumor-infiltrating lymphocyte and use thereof
AssigneesSuzhou Grit Biotechnology Co., Ltd., Zhuhai Tuoyu Biotechnology Co., Ltd., Shanghai Grit Biotechnology Co., Ltd., Zhuhai Grit Biotechnology Co., Ltd.
InventorsYarong LIU, Jingwei SUN, Jiahui JIN
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Abstract
Provided are modified tumor-infiltrating lymphocyte and the use thereof, in particular provided is a method for culturing the tumor-infiltrating lymphocyte (TIL), which comprises increasing the expression and/or enhancing the activity of at least one cytokine of the TIL. Also provided is a method for preventing and/or treating tumors by using the tumor-infiltrating lymphocyte.
Claims357
A method for culturing tumor-infiltrating lymphocytes (TILs), wherein the method comprising:
- improving the expression and/or enhancing the activity of at least one cytokine of the TILs, and co-culturing the TILs with feeder cells after contacting the TILs with T cell activators and/or T cell growth factors for a period of time.
A method for culturing tumor-infiltrating lymphocytes (TILs), wherein the method comprising:
- improving the expression and/or enhancing the activity of at least one cytokine of the TILs, wherein the TILs comprise TILs obtained by co-culturing with feeder cells after contacting the TILs with T cell activators and/or T cell growth factors for a period of time.
A method for culturing tumor-infiltrating lymphocytes (TILs), wherein the method comprising:
- co-culturing the TILs with feeder cells after contacting the TILs with T cell activators and/or T cell growth factors for a period of time, wherein the TILs comprise TTLs obtained by improving the expression and/or enhancing the activity of at least one cytokine of the TILs.
A method for culturing tumor-infiltrating lymphocytes (TILs), comprising:
- (A) contacting a first TIL population derived from tumor tissues and not expanded in vitro with T cell growth factors, wherein a second TIL population is obtained via the step (A);
- (B) improving the expression and/or enhancing the activity of at least one cytokine of the second TIL population, and co-culturing the second TIL population with feeder cells after contacting the second TIL population with T cell activators and/or T cell growth factors for a period of time, wherein a third TIL population is obtained via the step (B).
A method for culturing tumor-infiltrating lymphocytes (TILs), comprising:
- (A) resuscitating and/or continuing culturing an in vitro TIL population to obtain a second TIL population, wherein the in vitro TIL population comprises a TIL population obtained by in vitro expansion of a first TIL population derived from tumor tissues and not expanded in vitro;
- (B) improving the expression and/or enhancing the activity of at least one cytokine of the second TIL population, and co-culturing the second TIL population with feeder cells after contacting the second TIL population with T cell activators and/or T cell growth factors for a period of time, wherein a third TIL population is obtained via the step (B).
A method for culturing tumor-infiltrating lymphocytes (TILs), comprising:
- (A) contacting a first TIL population derived from tumor tissues and not expanded in vitro with T cell growth factors, wherein a second TIL population is obtained via the step (A);
- (B) improving the expression and/or enhancing the activity of at least one cytokine of the second TIL population, and contacting the second TIL population with T cell activators and/or T cell growth factors, wherein a third TIL population is obtained via the step (B);
- (C) co-culturing the third TIL population with feeder cells, wherein a fourth TIL population is obtained via the step (C).
A method for culturing tumor-infiltrating lymphocytes (TILs), comprising:
- (A) resuscitating and/or continuing culturing an in vitro TIL population to obtain a second TIL population, wherein the in vitro TIL population comprises a TIL population obtained by in vitro expansion of a first TIL population derived from tumor tissues and not expanded in vitro;
- (B) improving the expression and/or enhancing the activity of at least one cytokine of the second TIL population, and contacting the second TIL population with T cell activators and/or T cell growth factors, wherein a third TIL population is obtained via the step (B);
- (C) co-culturing the third TIL population with feeder cells, wherein a fourth TIL population is obtained via the step (C).
A method for culturing tumor-infiltrating lymphocytes (TILs), comprising:
- (A) contacting a first TIL population derived from tumor tissues and not expanded in vitro with T cell growth factors, wherein a second TIL population is obtained via the step (A);
- (B) contacting the second TIL population with T cell activators and/or T cell growth factors, wherein a third TIL population is obtained via the step (B);
- (C) improving the expression and/or enhancing the activity of at least one cytokine of the third TIL population, wherein a fourth TIL population is obtained via the step (C);
- (D) co-culturing the fourth TIL population with feeder cells, wherein a fifth TIL population is obtained via the step (D).
A method for culturing tumor-infiltrating lymphocytes (TILs), comprising:
- (A) resuscitating and/or continuing culturing an in vitro TIL population to obtain a second TIL population, wherein the in vitro TIL population comprises a TIL population obtained by in vitro expansion of a first TIL population derived from tumor tissues and not expanded in vitro;
- (B) contacting the second TIL population with T cell activators and/or T cell growth factors, wherein a third TIL population is obtained via the step (B);
- (C) improving the expression and/or enhancing the activity of at least one cytokine of the third TIL population, wherein a fourth TIL population is obtained via the step (C);
- (D) co-culturing the fourth TIL population with feeder cells, wherein a fifth TIL population is obtained via the step (D).
A method for culturing tumor-infiltrating lymphocytes (TILs), wherein the method comprising:
- improving the expression and/or enhancing the activity of at least one cytokine of the TILs, and contacting the TILs with a CD28 agonist.
A method for culturing tumor-infiltrating lymphocytes (TILs), wherein the method comprising:
- improving the expression and/or enhancing the activity of at least one cytokine of the TILs, wherein the TILs comprise TILs obtained by contacting the TTLs with a CD28 agonist.
A method for culturing tumor-infiltrating lymphocytes (TILs), wherein the method comprising:
- contacting the TTLs with a CD28 agonist, wherein the TILs comprise TILs obtained by improving the expression and/or enhancing the activity of at least one cytokine of the TILs.
A method for culturing tumor-infiltrating lymphocytes (TILs), comprising:
- (A) contacting a first TIL population derived from tumor tissues and not expanded in vitro with T cell growth factors, wherein a second TIL population is obtained via the step (A);
- (B) improving the expression and/or enhancing the activity of at least one cytokine of the second TIL population, and contacting the TTLs with a CD28 agonist, wherein a third TIL population is obtained via the step (B).
A method for culturing tumor-infiltrating lymphocytes (TILs), comprising:
- (A) resuscitating and/or continuing culturing an in vitro TIL population to obtain a second TIL population, wherein the in vitro TIL population comprises a TIL population obtained by in vitro expansion of a first TIL population derived from tumor tissues and not expanded in vitro;
- (B) improving the expression and/or enhancing the activity of at least one cytokine of the second TIL population, and contacting the TTLs with a CD28 agonist, wherein a third TIL population is obtained via the step (B).
Dependencies are inferred from the claim text.