Stabilized Pre-Fusion PIV3 F Proteins
AssigneeJanssen Vaccines & Prevention B.V.
InventorsJohannes Petrus Maria Langedijk, Mark Johannes Gerardus Bakkers, Tina Ritschel, Jaroslaw Juraszek
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Abstract
The present invention relates to stabilized pre-fusion human parainfluenza virus 3 (HPIV3) F protein, and fragments thereof. The invention also relates to nucleic acid molecules encoding such proteins and fragments, and to the use of the proteins, fragments and nucleic acid molecules.
Claims29
Stabilized pre-fusion human parainfluenza virus 3 (HPIV3) F protein, comprising an F1 and an F2 domain comprising an amino acid sequence of the F1 and F2 domain of an F protein of an HPIV3 strain, comprising a hydrophobic amino acid at position 470 and at position 477, wherein the numbering of the amino acid positions is according to the numbering is amino acid residues in SEQ ID NO:
- 1.
Protein according to any one of claims 1-4, wherein the amino acid residue at position 204 is D and/or the amino acid residue at position 367 is L and/or the amino acid residue at position 436 is P, and/or comprising a disulfide bridge between the amino acid residues 38 and 291.
Protein according to any one of the preceding claims, comprising a truncated F1 domain.
Protein according to claim 6, wherein the truncated F1 domain does not comprise the transmembrane and cytoplasmic regions.
Protein according to any one of claims 1-8, wherein a heterologous trimerization domain is linked to the truncated F1 domain.
Stabilized soluble pre-fusion human parainfluenza virus 3 (HPIV3) F protein, comprising a truncated F1 domain and an F2 domain, comprising an amino acid sequence of the F1 and F2 domain of an F protein of an HPIV3 strain, wherein the amino acid residue at position 470 and/or 477 is a hydrophobic amino acid, wherein the numbering of the amino acid positions is according to the numbering is amino acid residues in SEQ ID NO:
- 1.
Protein according to claim 10, wherein the hydrophobic amino acid at position 470 and at position 477 is valine (V).
Protein according to any of the preceding claims, comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
- 199, SEQ ID NO:
- 201, SEQ ID NO:
- 208, SEQ ID NO:
- 209 and SEQ ID NO:
- 210, or fragments thereof.
Dependencies are inferred from the claim text.