US 2024/0197849 A1 · IMMUNOGENIC COMPOSITIONS, ANTIGEN SCREENING METHODS, AND METHODS OF GENERATING IMMUNE RESPONSES

Abstract

An immunogenic composition is provided herein. The immunogenic compositions are used to identify and select immunogenic antigens that elicit immune responses in a subject and may be subsequently used in multi-antigen vaccine compositions against one or more diseases or conditions. According to some embodiments, the immunogenic composition may include a plurality of nucleic acid fragments or minigenes derived from a nucleic acid library, wherein each nucleic acid fragment encodes a different antigen or functional portion thereof, and wherein the different antigens or functional portions thereof are associated with one or more disease or condition. The immunogenic composition may also include a delivery medium loaded with the plurality of nucleic acid fragments and in some embodiments, the delivery medium is loaded with nucleic acid fragments in such a way that individual antigen presenting cells receive only a subset of the nucleic acids within a vaccine in order to minimize antigenic competition.

Claims6

Dependent claims

An immunogenic composition comprising:

two or more plasmid pools, including a first plasmid pool and a second plasmid pool;
where at least the first plasmid pool is loaded onto or into a first delivery medium and where at least the second plasmid pool is loaded onto or into a second delivery medium;
where the first and the second delivery media are loaded into a dosing container;
where the first plasmid pool comprises a first plurality of plasmids and where the second plasmid pool comprises a second plurality of plasmids different from the first plurality of plasmids;
where each plasmid in the two or more plasmid pools contains one or more nucleic-acid sequences encoding a protein, or proteins, or a protein fragment;
where the first and the second delivery media comprise (i) one or more types of particles, each smaller than the size of the cell receiving the particle, wherein the particles further comprise a lipid or other coating molecule around the plasmids, or (ii) micro-needles;
and
where at least the first and the second plasmid pools in the dosing container together contain more than one of the nucleic-acid sequences.

5 more elements

Dependencies are inferred from the claim text.

Immunogenic compositions, antigen screening methods, and methods of generating immune responses

Life of this patent

Immunogenic compositions, antigen screening methods, and methods of generating immune responses

Life of this patent

Abstract

Claims6