Back to results
US 2024/0197848 A1Published application
Therapeutic vaccine for treatment of diabetes type 1 in children, application of the cell sorter and the method of multiplying treg cells to produce therapeutic vaccine for treatment of diabetes type 1
AssigneeGdanski Uniwersytet Medyczny
InventorsPiotr Trzonkowski, Malgorzata Mysliwiec, Natalia Marek-Trzonkowska
Life of this patent
Estimating the term…
Abstract
The gist of the invention consists in the therapeutic vaccine for treatment of diabetes type 1 in children, which contains Treg cells CD3(+)CD4(+)CD25(high)CD127(−). Claimed too is the cell sorter used to produce the vaccine and the method of multiplying Treg cells in vitro.
Claims13
1-6. (canceled)
A method of expanding a population of CD3(+)CD4(+)CD25(high)CD127(−) autologous Treg cells comprising:
- providing CD3(+)CD4(+)CD25(high)CD127(−) autologous Treg cells,
- propagating the CD3(+)CD4(+)CD25(high)CD127(−) autologous Treg cells ex vivo in medium supplemented with interleukin-2 and autologous inactivated serum in the presence of artificial cells that present an antigen for 7-14 days to produce an expanded a population of CD3(+)CD4(+)CD25(high)CD127(−) autologous Treg cells.
Dependencies are inferred from the claim text.